A simple, high-throughput method of protein and label removal from extracellular vesicle samples.
Nanoscale
; 13(6): 3737-3745, 2021 Feb 18.
Article
in En
| MEDLINE
| ID: mdl-33544111
ABSTRACT
Evidence continues to increase of the clinical utility extracellular vesicles (EVs) as translational biomarkers. While a wide variety of EV isolation and purification methods have been implemented, few techniques are high-throughput and scalable for removing excess fluorescent reagents (e.g. dyes, antibodies). EVs are too small to be recovered from routine cell-processing procedures, such as filtration or centrifugation. The lack of suitable methods for removing unbound labels, especially in optical assays, is a major roadblock to accurate EV phenotyping and utilization of EV assays in a translational or clinical setting. Therefore, we developed a method for using a multi-modal resin, referred to as EV-Clean, to remove unbound labels from EV samples, and we demonstrate improvement in flow cytometric EV analysis with the use of this EV-Clean method.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Extracellular Vesicles
Language:
En
Journal:
Nanoscale
Year:
2021
Document type:
Article