Environmental salinity influences the branchial expression of TCR pathway related genes based on transcriptome of a catadromous fish.
Comp Biochem Physiol Part D Genomics Proteomics
; 38: 100815, 2021 06.
Article
in En
| MEDLINE
| ID: mdl-33610026
ABSTRACT
Environmental salinity not only affects the physiological processes such as osmoregulation and hormonal control, but also changes the immune system in fishes. Studies are limited in fish on the roles of the T cell receptor (TCR)-related genes in relation to changes in environmental salinity. A large group of salinity-challenged transcripts was obtained in gills of marbled eel (Anguilla marmorata). Moreover, bioinformatic ways were used to identify the enriched TCR pathway related genes which were significantly different expressed in fresh water (FW), brackish water (BW) and seawater (SW). Meanwhile, the RT-qPCR results were validated and consistent with the RNA-seq results. TCR a, TCR b, CD45, CD28, PI3K, LCK and LAT were up-regulated when the salinity increases in BW and SW, which connected with the related signaling pathways (Ras-MAPK and PKC pathway). CD4 and Zap70 were down-regulated when the salinity increases in BW and SW, which connected with the PLC pathway. The research offers a novel viewpoint to explore the immune pathways including the TCR pathway in fish based on transcriptome.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Antigen, T-Cell
/
Fish Proteins
/
Anguilla
Limits:
Animals
Language:
En
Journal:
Comp Biochem Physiol Part D Genomics Proteomics
Journal subject:
BIOLOGIA
/
GENETICA
Year:
2021
Document type:
Article
Affiliation country: