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Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells using the Sleeping Beauty Transposon System.
Johnen, Sandra; Harmening, Nina; Marie, Corinne; Scherman, Daniel; Izsvák, Zsuzsanna; Ivics, Zoltán; Walter, Peter; Thumann, Gabriele.
Affiliation
  • Johnen S; Department of Ophthalmology, University Hospital RWTH Aachen; sjohnen@ukaachen.de.
  • Harmening N; Experimental Ophthalmology, University of Geneva; Department of Ophthalmology, University Hospitals of Geneva.
  • Marie C; Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé; Chimie ParisTech, PSL Research University.
  • Scherman D; Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé.
  • Izsvák Z; Max Delbrück Center for Molecular Medicine in the Helmholtz Association.
  • Ivics Z; Division of Medical Biotechnology, Paul-Ehrlich-Institute.
  • Walter P; Department of Ophthalmology, University Hospital RWTH Aachen.
  • Thumann G; Experimental Ophthalmology, University of Geneva; Department of Ophthalmology, University Hospitals of Geneva.
J Vis Exp ; (168)2021 02 04.
Article in En | MEDLINE | ID: mdl-33616098
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Transposable Elements / Electroporation / Transgenes / Transposases / Retinal Pigment Epithelium Limits: Humans Language: En Journal: J Vis Exp Year: 2021 Document type: Article Country of publication:
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Transposable Elements / Electroporation / Transgenes / Transposases / Retinal Pigment Epithelium Limits: Humans Language: En Journal: J Vis Exp Year: 2021 Document type: Article Country of publication: