iTRAQ-based proteomic analysis of duck muscle related to lipid oxidation.

ABSTRACT

Lipid oxidation is the main cause of quality deterioration in meat and meat products. To facilitate the identification of candidate molecular biomarkers that are linked to lipid oxidation, we performed the proteomic analysis of duck muscle using isobaric tag for relative and absolute quantification (iTRAQ), followed by parallel reaction monitoring (PRM) to confirm the iTRAQ results. Pectoralis major muscles were divided into 2 groups in accordance with lipid oxidation, and iTRAQ-based analysis identified a total of 301 differentially expressed proteins, of which 15 proteins were examined by PRM assay. Proteins involved in lipid binding and metabolism, lipolysis, stress response, oxidative respiratory chain, and redox regulation were found to be differentially expressed between 2 groups and might affect lipid oxidation in muscles. The findings could contribute to the improved understanding of key proteins and processes engaged in lipid oxidation of meat.