Adipogenic function of tetranectin mediated by enhancing mitotic clonal expansion via ERK signaling.
BMB Rep
; 54(7): 374-379, 2021 Jul.
Article
in En
| MEDLINE
| ID: mdl-33691910
ABSTRACT
Tetranectin (TN), an adipogenic serum protein, enhances adipocyte differentiation, however, its functional mechanism has yet to be elucidated. In the present study, we investigated the adipogenic function of TN by using medium containing TN-depleted fetal bovine serum (TN-del-FBS) and recombinant mouse TN (mTN). The adipocyte differentiation of 3T3-L1 cells was significantly enhanced by mTN supplementation essentially at differentiation induction, which indicated a potential role of the protein in the early differentiation phase. The adipogenic effect of mTN was more significant with insulin in the differentiation induction cocktail, implicating their close functional relationship. mTN enhanced not only the proliferation of growing cells, but also mitotic clonal expansion (MCE) that is a prerequisite for adipocyte differentiation in the early phase. Consistently, mTN increased the phosphorylation of ERK in the early phase of adipocyte differentiation. Results of this study demonstrate that the adipogenic function of mTN is mediated by enhancing MCE via ERK signaling. [BMB Reports 2021; 54(7) 374-379].
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adipocytes
/
Lectins, C-Type
Limits:
Animals
Language:
En
Journal:
BMB Rep
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2021
Document type:
Article