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Proteomic reveals the influences of smoke-water and karrikinolide on the biosynthesis of salvianolic acids and lignins in Salvia miltiorrhiza hairy roots.
Sun, Hui; Ding, Weina; Duan, Wanying; Zhou, Jie; Guo, Lanping.
Affiliation
  • Sun H; School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
  • Ding W; School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
  • Duan W; School of Biological Science and Technology, University of Jinan, Jinan, 250022, China.
  • Zhou J; School of Biological Science and Technology, University of Jinan, Jinan, 250022, China. zhoujie8761@163.com.
  • Guo L; State Key Laboratory of Dao-Di Herbs, National Resource Center for Chinese Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Planta ; 253(5): 87, 2021 Apr 03.
Article in En | MEDLINE | ID: mdl-33811528
MAIN CONCLUSION: The proteins related to the biosynthesis of salvianolic acids and lignins were regulated by smoke-water and karrikinolide in Salvia miltiorrhiza hairy roots. The effects of smoke-water (SW) and karrikinolide (KAR1) on the biosynthesis of salvianolic acids and lignins in Salvia miltiorrhiza hairy roots have been studied using proteomic technology. The results showed that a total of 1290 and 1678 differentially expressed proteins were respectively obtained in SW and KAR1 comparing to the control. Bioinformatics analysis indicated the differentially expressed proteins responding to SW and KAR1 treatments mainly involved in macromolecule metabolic process, cell part, binding, etc., and most of the proteins were located at the cytoplasm and cell membrane, followed by nuclear. In addition, the proteins involved in salvianolic acids biosynthesis were up-regulated, including 4-coumarate-CoA ligase (EC 6.2.1.12) and shikimate O-hydroxycinnamoyl-transferase (EC 2.3.1.133). Enzymes involved in lignins biosynthesis were also identified, e.g. cinnamyl-alcohol dehydrogenase (EC 1.1.1.195) and peroxidase (EC 1.11.1.7). The results indicated that proteins related to the biosynthesis of salvianolic acids and lignins were regulated by SW and KAR1 in S. miltiorrhiza hairy roots. This study will enhance our understanding of the mechanism by which SW and KAR1 on the biosynthesis of salvianolic acids and lignins in S. miltiorrhiza hairy roots.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Salvia miltiorrhiza Language: En Journal: Planta Year: 2021 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Salvia miltiorrhiza Language: En Journal: Planta Year: 2021 Document type: Article Affiliation country: Country of publication: