Protocol for RNA-seq library preparation starting from a rare muscle stem cell population or a limited number of mouse embryonic stem cells.
STAR Protoc
; 2(2): 100451, 2021 06 18.
Article
in En
| MEDLINE
| ID: mdl-33937872
ABSTRACT
It remains challenging to generate reproducible, high-quality cDNA libraries from RNA derived from rare cell populations. Here, we describe a protocol for high-throughput RNA-seq library preparation, including isolation of 200 skeletal muscle stem cells from mouse tibialis anterior muscle by fluorescence-activated cell sorting and cDNA preparation. We also describe RNA extraction and cDNA preparation from differentiating mouse embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Juan et al. (2016) and Garcia-Prat et al. (2016).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Library
/
Myoblasts, Skeletal
/
Flow Cytometry
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Mouse Embryonic Stem Cells
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RNA-Seq
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2021
Document type:
Article
Affiliation country: