[CRISPR/Cas9 technology in disease research and therapy: a review].
Sheng Wu Gong Cheng Xue Bao
; 37(4): 1205-1228, 2021 Apr 25.
Article
in Zh
| MEDLINE
| ID: mdl-33973436
ABSTRACT
Genome editing is a genetic manipulation technique that can modify DNA sequences at the genome level, including insertion, knockout, replacement and point mutation of specific DNA fragments. The ultimate principle of genome editing technology relying on engineered nucleases is to generate double-stranded DNA breaks at specific locations in genome and then repair them through non-homologous end joining or homologous recombination. With the intensive study of these nucleases, genome editing technology develops rapidly. The most used nucleases include meganucleases, zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats associated Cas proteins. Based on introducing the development and principles of above mentioned genome editing technologies, we review the research progress of CRISPR/Cas9 system in the application fields of identification of gene function, establishment of disease model, gene therapy, immunotherapy and its prospect.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Clustered Regularly Interspaced Short Palindromic Repeats
/
CRISPR-Cas Systems
Language:
Zh
Journal:
Sheng Wu Gong Cheng Xue Bao
Journal subject:
BIOTECNOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
Country of publication:
CHINA
/
CN
/
REPUBLIC OF CHINA