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Comparative Proteomic Profiling: Cellular Metabolisms Are Mainly Affected in Senecavirus A-Inoculated Cells at an Early Stage of Infection.
Liu, Fuxiao; Ni, Bo; Wei, Rong.
Affiliation
  • Liu F; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
  • Ni B; Surveillance Laboratory of Livestock Diseases, China Animal Health and Epidemiology Center, Qingdao 266032, China.
  • Wei R; Surveillance Laboratory of Livestock Diseases, China Animal Health and Epidemiology Center, Qingdao 266032, China.
Viruses ; 13(6)2021 05 31.
Article in En | MEDLINE | ID: mdl-34072643
ABSTRACT
Senecavirus A (SVA), also known as Seneca Valley virus, belongs to the genus Senecavirus in the family Picornaviridae. SVA can cause vesicular disease and epidemic transient neonatal losses in pigs. This virus efficiently propagates in some non-pig-derived cells, like the baby hamster kidney (BHK) cell line and its derivate (BSR-T7/5). Conventionally, a few proteins or only one protein is selected for exploiting a given mechanism concerning cellular regulation after SVA infection in vitro. Proteomics plays a vital role in the analysis of protein profiling, protein-protein interactions, and protein-directed metabolisms, among others. Tandem mass tag-labeled liquid chromatography-tandem mass spectrometry combined with the parallel reaction monitoring technique is increasingly used for proteomic research. In this study, this combined method was used to uncover separately proteomic profiles of SVA- and non-infected BSR-T7/5 cells. Furthermore, both proteomic profiles were compared with each other. The proteomic profiling showed that a total of 361 differentially expressed proteins were identified, out of which, 305 and 56 were upregulated and downregulated in SVA-infected cells at 12 h post-inoculation, respectively. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses showed that cellular metabolisms were affected mainly in SVA-inoculated cells at an early stage of infection. Therefore, an integrated metabolic atlas remains to be explored via metabolomic methods.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Picornaviridae / Proteomics / Metabolic Networks and Pathways Type of study: Prognostic_studies Limits: Animals Language: En Journal: Viruses Year: 2021 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Picornaviridae / Proteomics / Metabolic Networks and Pathways Type of study: Prognostic_studies Limits: Animals Language: En Journal: Viruses Year: 2021 Document type: Article Affiliation country: