SARS-CoV-2 infection induces beta cell transdifferentiation.
Cell Metab
; 33(8): 1577-1591.e7, 2021 08 03.
Article
in En
| MEDLINE
| ID: mdl-34081913
ABSTRACT
Recent clinical data have suggested a correlation between coronavirus disease 2019 (COVID-19) and diabetes. Here, we describe the detection of SARS-CoV-2 viral antigen in pancreatic beta cells in autopsy samples from individuals with COVID-19. Single-cell RNA sequencing and immunostaining from ex vivo infections confirmed that multiple types of pancreatic islet cells were susceptible to SARS-CoV-2, eliciting a cellular stress response and the induction of chemokines. Upon SARS-CoV-2 infection, beta cells showed a lower expression of insulin and a higher expression of alpha and acinar cell markers, including glucagon and trypsin1, respectively, suggesting cellular transdifferentiation. Trajectory analysis indicated that SARS-CoV-2 induced eIF2-pathway-mediated beta cell transdifferentiation, a phenotype that could be reversed with trans-integrated stress response inhibitor (trans-ISRIB). Altogether, this study demonstrates an example of SARS-CoV-2 infection causing cell fate change, which provides further insight into the pathomechanisms of COVID-19.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Insulin-Secreting Cells
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Cell Transdifferentiation
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SARS-CoV-2
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COVID-19
Limits:
Adolescent
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Adult
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Aged
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Aged80
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Animals
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Female
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Humans
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Male
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Middle aged
Language:
En
Journal:
Cell Metab
Journal subject:
METABOLISMO
Year:
2021
Document type:
Article
Affiliation country: