Synthetic extracellular matrices with tailored adhesiveness and degradability support lumen formation during angiogenic sprouting.
Nat Commun
; 12(1): 3402, 2021 06 07.
Article
in En
| MEDLINE
| ID: mdl-34099677
ABSTRACT
A major deficit in tissue engineering strategies is the lack of materials that promote angiogenesis, wherein endothelial cells from the host vasculature invade the implanted matrix to form new blood vessels. To determine the material properties that regulate angiogenesis, we have developed a microfluidic in vitro model in which chemokine-guided endothelial cell sprouting into a tunable hydrogel is followed by the formation of perfusable lumens. We show that long, perfusable tubes only develop if hydrogel adhesiveness and degradability are fine-tuned to support the initial collective invasion of endothelial cells and, at the same time, allow for matrix remodeling to permit the opening of lumens. These studies provide a better understanding of how cell-matrix interactions regulate angiogenesis and, therefore, constitute an important step towards optimal design criteria for tissue-engineered materials that require vascularization.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neovascularization, Physiologic
/
Hydrogels
/
Tissue Engineering
/
Extracellular Matrix
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Nat Commun
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2021
Document type:
Article
Affiliation country: