Your browser doesn't support javascript.
loading
Impact of photobiomodulation in a patient-derived xenograft model of oral squamous cell carcinoma.
Silveira, Felipe Martins; Schmidt, Tuany Rafaeli; Neumann, Bruna; Rosset, Clévia; Zanella, Virgilio Gonzales; Maahs, Gerson Schulz; Martins, Marco Antonio Trevizani; Arany, Praveen; Wagner, Vivian Petersen; Lopes, Márcio Ajudarte; Santos-Silva, Alan Roger; Martins, Manoela Domingues.
Affiliation
  • Silveira FM; Oral Diagnosis Department, Piracicaba Dental School, University of Campinas, Campinas, Brazil.
  • Schmidt TR; Experimental Pathology Unit, Porto Alegre Clinics Hospital, Porto Alegre, Brazil.
  • Neumann B; Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Rosset C; Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Zanella VG; Laboratory Research Unit, Experimental Research Center, Porto Alegre Clinics Hospital, Porto Alegre, Brazil.
  • Maahs GS; Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Martins MAT; Head and Neck Surgery Department, Santa Rita Hospital, Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, Brazil.
  • Arany P; Division of Otorhinolaryngology, Porto Alegre Clinics Hospital, Porto Alegre, Brazil.
  • Wagner VP; Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
  • Lopes MA; Department of Oral Medicine, Porto Alegre Clinics Hospital, Porto Alegre, Brazil.
  • Santos-Silva AR; Departments of Oral Biology and Biomedical Engineering, Schools of Dental Medicine, Engineering and Applied Sciences, State University of New York at Buffalo, Buffalo, NY, USA.
  • Martins MD; Oral Diagnosis Department, Piracicaba Dental School, University of Campinas, Campinas, Brazil.
Oral Dis ; 29(2): 547-556, 2023 Mar.
Article in En | MEDLINE | ID: mdl-34273227
ABSTRACT

BACKGROUND:

Photobiomodulation therapy (PBMT) is an effective method for the prevention of oral mucositis. However, the effects of PBMT on oral squamous cell carcinoma (OSCC) have not yet been fully elucidated. This study aimed to evaluate the impact of PBMT in an OSCC-patient-derived xenograft (OSCC-PDX) model.

METHODS:

BALB/c nude mice with OSCC-PDX models were divided into Control, without PBMT (n = 8); Immediate irradiation, PBMT since one week after tumor implantation (n = 6); and Late irradiation, PBMT after tumors reached 200 mm3 (n = 6). OSCC-PDX were daily irradiated (660 nm; 100 mW; 6 J/cm2 ; 0,2 J/point) for 12 weeks. The tumors were collected and submitted to volumetric, histological, immunohistochemistry, and cell cycle analysis.

RESULTS:

No significant differences in the volumetric measurements (p = 0.89) and in the histopathological grade (p > 0.05) were detected between the groups. The immunohistochemical analysis of Ki-67 (p = 0.9661); H3K9ac (p = 0.3794); and BMI1 (p = 0.5182), and the evaluation of the cell cycle phases (p > 0.05) by flow cytometry also did not demonstrate significant differences between the irradiated and non-irradiated groups.

CONCLUSION:

In this study, PBMT did not impact the behavior of OSCC-PDX models. This is an important preclinical outcome regarding safety concerns of the use of PBMT in cancer patients.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mouth Neoplasms / Carcinoma, Squamous Cell / Low-Level Light Therapy / Head and Neck Neoplasms Limits: Animals / Humans Language: En Journal: Oral Dis Journal subject: ODONTOLOGIA Year: 2023 Document type: Article Affiliation country:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mouth Neoplasms / Carcinoma, Squamous Cell / Low-Level Light Therapy / Head and Neck Neoplasms Limits: Animals / Humans Language: En Journal: Oral Dis Journal subject: ODONTOLOGIA Year: 2023 Document type: Article Affiliation country: