Anti-glycation activities of methyl gallate in vitro and in human explants.
J Cosmet Dermatol
; 21(6): 2602-2609, 2022 Jun.
Article
in En
| MEDLINE
| ID: mdl-34418257
ABSTRACT
BACKGROUND:
The build-up of advanced glycation end products (AGEs) is one of important factor of skin aging. Natural compounds with anti-glycation activities might have great anti-aging potential.AIMS:
The objective of this study was to evaluate an anti-glycation effects of methyl gallate as a potent ingredient for anti-aging.METHODS:
We first evaluated the AGEs inhibitory ability of methyl gallate in BSA/glucose system. Levels of Nε-CML and carbonyl contents were also measured in BSA/glucose system. To further investigate if methyl gallate could prevent glycation in full-thickness human skin explants. Glycation action was determined by the observation of the general morphology of dermis and epidermis structures and FBN-1 and of CML immunostaining. In an in-vivo study, primary irritation test was also performed to ensure the safety of methyl gallate for human skin.RESULTS:
It is known that methyl gallate can suppress glycation reaction between BSA and glucose. Methyl gallate also has a remarkable potential to reduce the oxidation of proteins. Furthermore, the anti-glycation activity of methyl gallate has been confirmed in a human skin ex-vivo model. Methyl gallate decreased the expression of CML but stimulated the expression of FBN-1 compared with MGO treatment. In an in-vivo study, methyl gallate (0.1%) did not cause any skin irritation, suggesting that methyl gallate could be used as an active ingredient in cosmetics.CONCLUSION:
Our results showed that methyl gallate could protect against glucose-mediated glycation in vitro. Furthermore, methyl gallate significantly prevented glycation in living human skin explants. Due to these beneficial effects, methyl gallate can be used to prevent or manage AGE-mediated skin aging.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin Aging
/
Gallic Acid
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Cosmet Dermatol
Journal subject:
DERMATOLOGIA
Year:
2022
Document type:
Article