Your browser doesn't support javascript.
loading
Restoring mammary gland structures and functions with autogenous cell therapy.
Zhang, Dandan; Wang, Guodong; Qin, Liangshan; Liu, Quanhui; Zhu, Shaoqian; Ye, Sheng; Li, Xiaobo; Wu, Yulian; Hu, Yanan; Liu, Shulin; Jiao, Yafei; Sun, Longfei; Lv, Danwei; Ma, Jiawen; Luo, Man; Yao, Mengcheng; Li, Mengmei; Zhou, Lei; Pei, Surui; Li, Lanyu; Shi, Deshun; Huang, Ben.
Affiliation
  • Zhang D; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Wang G; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Qin L; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Liu Q; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Zhu S; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Ye S; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Li X; Annoroad Gene Technology (Beijing) Co., Ltd, Beijing, 100176, China.
  • Wu Y; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Hu Y; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Liu S; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Jiao Y; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Sun L; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Lv D; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Ma J; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Luo M; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Yao M; Annoroad Gene Technology (Beijing) Co., Ltd, Beijing, 100176, China.
  • Li M; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Zhou L; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China.
  • Pei S; Annoroad Gene Technology (Beijing) Co., Ltd, Beijing, 100176, China.
  • Li L; Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, the Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, China.
  • Shi D; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: ardsshi@gxu.edu.cn.
  • Huang B; State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, Guangxi, 530004, China; School of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530004, China. Electronic address: benhuang@gxu.edu.cn.
Biomaterials ; 277: 121075, 2021 10.
Article in En | MEDLINE | ID: mdl-34428734
In somatic cell reprogramming, cells must escape the somatic cell-specific gene expression program to adopt other cell fates. Here, in vitro chemical induction with RepSox generated chemically induced mammary epithelial cells (CiMECs) with milk secreting functions from goat ear fibroblasts (GEFs). Transplanted CiMECs regenerated the normal mammary gland structure with milk-secreting functions in nude mice. Single-cell RNA sequencing revealed that during the reprogramming process, GEFs may sequentially undergo embryonic ectoderm (EE)-like and different MEC developmental states and finally achieve milk secreting functions, bypassing the pluripotent state. Mechanistically, Smad3 upregulation induced by transforming growth factor ß (TGFß) receptor 1 (TGFßR1) downregulation led to GEF reprogramming into CiMECs without other reprogramming factors. The TGFßR1-Smad3 regulatory effects will provide new insight into the TGFß signaling pathway regulation of somatic cell reprogramming. These findings suggest an innovative strategy for autogenous cell therapy for mammary gland defects and the production of transgenic mammary gland bioreactors.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Goats / Mammary Glands, Animal Limits: Animals Language: En Journal: Biomaterials Year: 2021 Document type: Article Affiliation country: Country of publication:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Goats / Mammary Glands, Animal Limits: Animals Language: En Journal: Biomaterials Year: 2021 Document type: Article Affiliation country: Country of publication: