Engineered 3D vessel-on-chip using hiPSC-derived endothelial- and vascular smooth muscle cells.
Stem Cell Reports
; 16(9): 2159-2168, 2021 09 14.
Article
in En
| MEDLINE
| ID: mdl-34478648
ABSTRACT
Crosstalk between endothelial cells (ECs) and pericytes or vascular smooth muscle cells (VSMCs) is essential for the proper functioning of blood vessels. This balance is disrupted in several vascular diseases but there are few experimental models which recapitulate this vascular cell dialogue in humans. Here, we developed a robust multi-cell type 3D vessel-on-chip (VoC) model based entirely on human induced pluripotent stem cells (hiPSCs). Within a fibrin hydrogel microenvironment, the hiPSC-derived vascular cells self-organized to form stable microvascular networks reproducibly, in which the vessels were lumenized and functional, responding as expected to vasoactive stimulation. Vascular organization and intracellular Ca2+ release kinetics in VSMCs could be quantified using automated image analysis based on open-source software CellProfiler and ImageJ on widefield or confocal images, setting the stage for use of the platform to study vascular (patho)physiology and therapy.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tissue Engineering
/
Myocytes, Smooth Muscle
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Endothelial Cells
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Induced Pluripotent Stem Cells
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Lab-On-A-Chip Devices
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Cell Culture Techniques, Three Dimensional
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Stem Cell Reports
Year:
2021
Document type:
Article
Affiliation country: