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A novel ratiometric MALDI-MS quantitation strategy for alkaline phosphatase activity with a homogeneous reaction and a tunable dynamic range.
Ma, Rong-Na; Zhang, Min; Hu, Chao-Long; Pan, Hui-Jing; Si, Lei; Wang, Huaisheng.
Affiliation
  • Ma RN; School of Chemistry and Chemical Engineering, Shandong Provincial Key Laboratory/Collaborative Innovation Center of Chemical Energy Storage, Liaocheng University, Liaocheng 252059, Shandong, P. R. China. marongna@lcu.edu.cn.
  • Zhang M; School of Chemistry and Chemical Engineering, Shandong Provincial Key Laboratory/Collaborative Innovation Center of Chemical Energy Storage, Liaocheng University, Liaocheng 252059, Shandong, P. R. China. marongna@lcu.edu.cn.
  • Hu CL; School of Chemistry and Chemical Engineering, Shandong Provincial Key Laboratory/Collaborative Innovation Center of Chemical Energy Storage, Liaocheng University, Liaocheng 252059, Shandong, P. R. China. marongna@lcu.edu.cn.
  • Pan HJ; School of Chemistry and Chemical Engineering, Shandong Provincial Key Laboratory/Collaborative Innovation Center of Chemical Energy Storage, Liaocheng University, Liaocheng 252059, Shandong, P. R. China. marongna@lcu.edu.cn.
  • Si L; Department of Clinical Laboratory, Liaocheng People's Hospital, Shandong First Medical University, Liaocheng 252000, Shandong, P. R. China.
  • Wang H; School of Chemistry and Chemical Engineering, Shandong Provincial Key Laboratory/Collaborative Innovation Center of Chemical Energy Storage, Liaocheng University, Liaocheng 252059, Shandong, P. R. China. marongna@lcu.edu.cn.
Chem Commun (Camb) ; 57(71): 8885-8888, 2021 Sep 06.
Article in En | MEDLINE | ID: mdl-34486626
ABSTRACT
A unique ratiometric MALDI-MS strategy is proposed for the convenient and reliable quantitation of alkaline phosphatase based on the homogeneous enzymatic cleavage of a coded phosphopeptide (CPP)-triggered double-signal output. The dynamic range can be tuned by simply adjusting the primary concentration of CPP. The proposed strategy is also capable of being challenged by real human serum, and thus it may offer a wonderful approach for the convenient identification and quantitation of various enzyme activities in clinical diagnosis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Alkaline Phosphatase Limits: Humans Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2021 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Alkaline Phosphatase Limits: Humans Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2021 Document type: Article