A novel ratiometric MALDI-MS quantitation strategy for alkaline phosphatase activity with a homogeneous reaction and a tunable dynamic range.
Chem Commun (Camb)
; 57(71): 8885-8888, 2021 Sep 06.
Article
in En
| MEDLINE
| ID: mdl-34486626
ABSTRACT
A unique ratiometric MALDI-MS strategy is proposed for the convenient and reliable quantitation of alkaline phosphatase based on the homogeneous enzymatic cleavage of a coded phosphopeptide (CPP)-triggered double-signal output. The dynamic range can be tuned by simply adjusting the primary concentration of CPP. The proposed strategy is also capable of being challenged by real human serum, and thus it may offer a wonderful approach for the convenient identification and quantitation of various enzyme activities in clinical diagnosis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Alkaline Phosphatase
Limits:
Humans
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2021
Document type:
Article