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A Method of Direct Quantitation of Lactobacillus spp. in Intestinal Contents Based on Real-Time PCR.
Markova, Yu M; Stetsenko, V V; Polyanina, A S.
Affiliation
  • Markova YM; Federal Research Center of Nutrition, Biotechnology, and Food Safety, Moscow, Russia. yulia.markova.ion@gmail.com.
  • Stetsenko VV; Federal Research Center of Nutrition, Biotechnology, and Food Safety, Moscow, Russia.
  • Polyanina AS; Federal Research Center of Nutrition, Biotechnology, and Food Safety, Moscow, Russia.
Bull Exp Biol Med ; 171(6): 794-797, 2021 Oct.
Article in En | MEDLINE | ID: mdl-34709511
ABSTRACT
The method of direct quantitation of Lactobacillus spp. and L. acidophilus in intestinal contents based on real-time PCR was developed. It does not require culturing and allows estimating the number of living lactobacilli cells (measured in lg CFU) in absolute quantitative PCR format.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Ribosomal, 16S / Polymerase Chain Reaction / Lactobacillus plantarum / Gastrointestinal Microbiome / Lacticaseibacillus paracasei / Lactobacillus acidophilus Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Bull Exp Biol Med Year: 2021 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Ribosomal, 16S / Polymerase Chain Reaction / Lactobacillus plantarum / Gastrointestinal Microbiome / Lacticaseibacillus paracasei / Lactobacillus acidophilus Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Bull Exp Biol Med Year: 2021 Document type: Article Affiliation country: