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A method for rapid flow-cytometric isolation of endothelial nuclei and RNA from archived frozen brain tissue.
Kimble, Amy L; Silva, Jordan; Omar, Omar M; Murphy, Melissa; Hensel, Jessica A; Nicholas, Sarah-Anne E; Jellison, Evan R; Reese, Bo; Murphy, Patrick A.
Affiliation
  • Kimble AL; Center for Vascular Biology, UCONN Health, Farmington, CT, USA.
  • Silva J; Center for Vascular Biology, UCONN Health, Farmington, CT, USA.
  • Omar OM; Center for Vascular Biology, UCONN Health, Farmington, CT, USA.
  • Murphy M; Center for Vascular Biology, UCONN Health, Farmington, CT, USA.
  • Hensel JA; Center for Vascular Biology, UCONN Health, Farmington, CT, USA.
  • Nicholas SE; Center for Vascular Biology, UCONN Health, Farmington, CT, USA.
  • Jellison ER; Department of Immunology, UCONN Health, Farmington, CT, USA.
  • Reese B; Institute for Systems Genomics-Center for Genome Innovation, UCONN, Storrs, CT, USA.
  • Murphy PA; Center for Vascular Biology, UCONN Health, Farmington, CT, USA. pamurphy@uchc.edu.
Lab Invest ; 102(2): 204-211, 2022 02.
Article in En | MEDLINE | ID: mdl-34775494

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain / RNA / Cell Nucleus / Single-Cell Analysis / Human Umbilical Vein Endothelial Cells / Flow Cytometry Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Lab Invest Year: 2022 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain / RNA / Cell Nucleus / Single-Cell Analysis / Human Umbilical Vein Endothelial Cells / Flow Cytometry Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Lab Invest Year: 2022 Document type: Article Affiliation country: Country of publication: