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Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis.
Dang, Mai T; Mafra, Fernanda; Haldar, Malay.
Affiliation
  • Dang MT; Division of Neurology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
  • Mafra F; Division of Pediatric and Developmental Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA.
  • Haldar M; Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
STAR Protoc ; 2(4): 100957, 2021 12 17.
Article in En | MEDLINE | ID: mdl-34825218
Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellum without cell sorting to minimize cell damage for scRNA-seq. This protocol includes the procedures for further enrichment of myeloid cells using CD11b+ magnetic beads, followed by the generation of scRNA library and sequencing analysis. For complete details on the use and execution of this protocol, please refer to Dang et al. (2021).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain Neoplasms / Cell Separation / Sequence Analysis, RNA / Myeloid Cells / Single-Cell Analysis Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain Neoplasms / Cell Separation / Sequence Analysis, RNA / Myeloid Cells / Single-Cell Analysis Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Document type: Article Affiliation country: Country of publication: