GDF9 concentration in embryo culture medium is linked to human embryo quality and viability.
J Assist Reprod Genet
; 39(1): 117-125, 2022 Jan.
Article
in En
| MEDLINE
| ID: mdl-34845575
ABSTRACT
PURPOSE:
We aimed to evaluate the link between the GDF9 concentration in day 3 human embryo culture medium and embryo quality and viability.METHODS:
Two independent, prospective, observational studies were conducted. In study 1, a total of 280 embryos from 70 patients who obtained at least 4 embryos with 6-10 blastomeres (2 transferable and 2 non-transferable embryos) at day 3 were enrolled. In study 2, a total of 119 embryos from 61 patients (29 fully implanted and 32 non-implanted patients) were enrolled. The corresponding GDF9 concentrations in spent culture medium of embryos were quantified by ELISA assay. The expression pattern of GDF9 in human embryos was investigated using Q-PCR and immunofluorescence.RESULTS:
GDF9 mRNA and protein were detected from human oocytes to eight-cell embryos and displayed a slow decreasing trend. In study 1, GDF9 concentration in culture medium is lower for transferable embryos compared with non-transferable embryos (331 pg/mL (quartiles 442, 664 pg/mL) vs. 518 pg/mL (quartiles 328, 1086 pg/mL), P < 0.001), and increased commensurate with the diminution of the embryo quality (P < 0.001). In study 2, significantly lower GDF9 concentration was detected for implanted embryos than non-implanted embryos (331 pg/mL (quartiles 156, 665 pg/mL) vs. 518 pg/mL (quartiles 328, 1086 pg/mL), P < 0.001). The same trend was found between the embryos that led to live birth and those that failed.CONCLUSION:
The GDF9 concentration in culture medium is linked to embryo quality and viability, and exhibited the potential to be a non-invasive biomarker for embryo selection.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Culture Media
/
Embryonic Development
/
Growth Differentiation Factor 9
Type of study:
Observational_studies
Limits:
Adult
/
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
J Assist Reprod Genet
Journal subject:
GENETICA
/
MEDICINA REPRODUTIVA
Year:
2022
Document type:
Article
Affiliation country: