Investigating the effects of compound paralogous EPHB receptor mutations on mouse facial development.
Dev Dyn
; 251(7): 1138-1155, 2022 07.
Article
in En
| MEDLINE
| ID: mdl-35025117
ABSTRACT
BACKGROUND:
Variation in facial shape may arise from the combinatorial or overlapping actions of paralogous genes. Given its many members, and overlapping expression and functions, the EPH receptor family is a compelling candidate source of craniofacial morphological variation. We performed a detailed morphometric analysis of an allelic series of E14.5 Ephb1-3 receptor mutants to determine the effect of each paralogous receptor gene on craniofacial morphology.RESULTS:
We found that Ephb1, Ephb2, and Ephb3 genotypes significantly influenced facial shape, but Ephb1 effects were weaker than Ephb2 and Ephb3 effects. Ephb2-/- and Ephb3-/- mutations affected similar aspects of facial morphology, but Ephb3-/- mutants had additional facial shape effects. Craniofacial differences across the allelic series were largely consistent with predicted additive genetic effects. However, we identified a potentially important nonadditive effect where Ephb1 mutants displayed different morphologies depending on the combination of other Ephb paralogs present, where Ephb1+/- , Ephb1-/- , and Ephb1-/- ; Ephb3-/- mutants exhibited a consistent deviation from their predicted facial shapes.CONCLUSIONS:
This study provides a detailed assessment of the effects of Ephb receptor gene paralogs on E14.5 mouse facial morphology and demonstrates how the loss of specific receptors contributes to facial dysmorphology.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Eph Family
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Receptor, EphB1
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Receptor, EphB3
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Ephrin-B1
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Maxillofacial Development
Limits:
Animals
Language:
En
Journal:
Dev Dyn
Journal subject:
ANATOMIA
Year:
2022
Document type:
Article
Affiliation country: