Chitosan-collagen-hydroxyapatite membranes for tissue engineering.
J Mater Sci Mater Med
; 33(2): 18, 2022 Jan 24.
Article
in En
| MEDLINE
| ID: mdl-35072812
ABSTRACT
Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues. The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent years. Chitosan, calcium phosphates collagen, and their combination as composite materials fulfill the required properties and could result in biostimulation for tissue regeneration. In the present work, the chitosan/collagen/hydroxyapatite membranes were prepared with different concentrations of collagen and hydroxyapatite. Cell adhesion was evaluated by MTS assay for two in vitro models. Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out. The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA). The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content. All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering. Graphical abstract.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Collagen
/
Durapatite
/
Tissue Engineering
/
Chitosan
/
Mesenchymal Stem Cells
Limits:
Humans
Language:
En
Journal:
J Mater Sci Mater Med
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2022
Document type:
Article
Affiliation country: