Cryo-ET of Env on intact HIV virions reveals structural variation and positioning on the Gag lattice.
Cell
; 185(4): 641-653.e17, 2022 02 17.
Article
in En
| MEDLINE
| ID: mdl-35123651
ABSTRACT
HIV-1 Env mediates viral entry into host cells and is the sole target for neutralizing antibodies. However, Env structure and organization in its native virion context has eluded detailed characterization. Here, we used cryo-electron tomography to analyze Env in mature and immature HIV-1 particles. Immature particles showed distinct Env positioning relative to the underlying Gag lattice, providing insights into long-standing questions about Env incorporation. A 9.1-Å sub-tomogram-averaged reconstruction of virion-bound Env in conjunction with structural mass spectrometry revealed unexpected features, including a variable central core of the gp41 subunit, heterogeneous glycosylation between protomers, and a flexible stalk that allows Env tilting and variable exposure of neutralizing epitopes. Together, our results provide an integrative understanding of HIV assembly and structural variation in Env antigen presentation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virion
/
Cryoelectron Microscopy
/
Gag Gene Products, Human Immunodeficiency Virus
/
Env Gene Products, Human Immunodeficiency Virus
/
Electron Microscope Tomography
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Cell
Year:
2022
Document type:
Article
Affiliation country: