HIV-1 Tat and cocaine coexposure impacts piRNAs to affect astrocyte energy metabolism.
Epigenomics
; 14(5): 261-278, 2022 03.
Article
in En
| MEDLINE
| ID: mdl-35170353
ABSTRACT
Aim:
To understand the effect of HIV infection and cocaine exposure on piRNA expression in human primary astrocytes. Materials &methods:
We used small RNA sequencing analysis to investigate the impacts of HIV-1 Tat and cocaine coexposure on the expression of piRNAs in human primary astrocytes.Results:
We identified 27,700 piRNAs and analyzed them by small RNA next-generation sequencing. A total of 239 piRNAs were significantly altered by HIV-1 Tat and cocaine coexposure. We also identified PIWIL1, PIWIL2, PIWIL3 and PIWIL4 as interacting partners of piRNAs that were affected by cocaine and HIV-1 Tat coexposure. Epigenetic changes in the expression levels of these piRNA targets were associated with Kyoto Encyclopedia of Genes and Genomes pathways of energy metabolism and neurodegeneration.Conclusion:
These findings provide evidence that cocaine exposure and HIV infection affect the expression levels of piRNA, PIWIL1, PIWIL2, PIWIL3 and PIWIL4.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
HIV Infections
/
HIV-1
/
Cocaine
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Epigenomics
Year:
2022
Document type:
Article
Affiliation country: