New spirit of an old technique: Characterization of lipid phase transitions via UV/Vis spectroscopy.
Spectrochim Acta A Mol Biomol Spectrosc
; 272: 121013, 2022 May 05.
Article
in En
| MEDLINE
| ID: mdl-35176647
ABSTRACT
One of the advantages of investigating lipid phase transitions by thermoanalytical techniques such as DSC is manifested in the proportionality of the signal strength on a DSC curve, attributed to a particular thermotropic event, and its cooperativity degree. Accordingly, the pretransition of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) is less noticeable than its main phase transition; as a matter of fact, when DSC measurements are performed at low heating rate, such low-cooperativity phase transition could go (almost) unnoticed. The aim of this work is to present temperature-dependent UV/Vis spectroscopy, based on a temperature-dependent change in DPPC suspension turbidity, as a technique applicable for determination of lipid phase transition temperatures. Multivariate analyzes of the acquired UV/Vis spectra show that phase transitions of the low-cooperativity degree, such as pretransitions, can be identified with the same certainty as transitions of a high-cooperativity degree.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
1,2-Dipalmitoylphosphatidylcholine
/
Lipid Bilayers
Language:
En
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
Journal subject:
BIOLOGIA MOLECULAR
Year:
2022
Document type:
Article
Affiliation country: