Revealing new biology from multiplexed, metal-isotope-tagged, single-cell readouts.
Trends Cell Biol
; 32(6): 501-512, 2022 06.
Article
in En
| MEDLINE
| ID: mdl-35181197
ABSTRACT
Mass cytometry (MC) is a recent technology that pairs plasma-based ionization of cells in suspension with time-of-flight (TOF) mass spectrometry to sensitively quantify the single-cell abundance of metal-isotope-tagged affinity reagents to key proteins, RNA, and peptides. Given the ability to multiplex readouts (~50 per cell) and capture millions of cells per experiment, MC offers a robust way to assay rare, transitional cell states that are pertinent to human development and disease. Here, we review MC approaches that let us probe the dynamics of cellular regulation across multiple conditions and sample types in a single experiment. Additionally, we discuss current limitations and future extensions of MC as well as computational tools commonly used to extract biological insight from single-cell proteomic datasets.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteomics
/
Isotopes
Limits:
Humans
Language:
En
Journal:
Trends Cell Biol
Year:
2022
Document type:
Article
Affiliation country: