Sperm mitochondrial DNA copy number in relation to semen quality: A cross-sectional study of 1164 potential sperm donors.
BJOG
; 129(13): 2098-2106, 2022 12.
Article
in En
| MEDLINE
| ID: mdl-35274799
ABSTRACT
OBJECTIVE:
To investigate the association between mitochondrial DNA copy number (mtDNAcn) and semen quality.DESIGN:
A cross-sectional study.SETTING:
Hubei Province Human Sperm Bank of China (from April 2017 to July 2018). POPULATION A total of 1164 healthy male sperm donors with 5739 specimens. MAIN OUTCOMEMEASURES:
Real-time quantitative polymerase chain reaction (RT-PCR) was used to measure sperm mtDNAcn. We also determined semen volume, concentration and motility parameters (progressive motility, nonprogressive motility and immotility).METHODS:
Mixed-effect models and general linear models were uses.RESULTS:
After adjusting for relevant confounding factors, mixed-effect models revealed diminished sperm motility (progressive and total), concentration, and total count across the quartiles of mtDNAcn (all P < 0.05). Compared with men in the lowest quartile, men in the highest quartile of mtDNAcn had lower progressive sperm motility, total motility, concentration and total count of -8.9% (95% CI -12.7% to -5.0%), -8.0% (95% CI -11.6% to -4.4%), -42.8% (95% CI -47.7% to -37.4%), and - 44.3% (95% CI -50.1% to -37.7%), respectively. These inverse dose-response relationships were further confirmed in the cubic spline models, where mtDNAcn was modelled as a continuous variable.CONCLUSIONS:
We found that mtDNAcn was inversely associated with semen quality in a dose-dependent manner. Our results provide novel clues that sperm mtDNAcn may serve as a useful predictor of human semen characteristics. TWEETABLE ABSTRACT Sperm mitochondrial DNA copy number was markedly associated with diminished sperm motility (progressive and total), concentration and total count.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sperm Motility
/
Semen Analysis
Type of study:
Observational_studies
/
Prevalence_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Humans
/
Male
Language:
En
Journal:
BJOG
Journal subject:
GINECOLOGIA
/
OBSTETRICIA
Year:
2022
Document type:
Article
Affiliation country: