Isolation and characterization of lytic bacteriophages from sewage at an egyptian tertiary care hospital against methicillin-resistant <i>Staphylococcus aureus</i> clinical isolates.

Samir, Safia; El-Far, Amira; Okasha, Hend; Mahdy, Rania; Samir, Fatima; Nasr, Sami

Isolation and characterization of lytic bacteriophages from sewage at an egyptian tertiary care hospital against methicillin-resistant Staphylococcus aureus clinical isolates.

Samir, Safia; El-Far, Amira; Okasha, Hend; Mahdy, Rania; Samir, Fatima; Nasr, Sami.

Affiliation

Samir S; Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt.
El-Far A; Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt.
Okasha H; Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt.
Mahdy R; Microbiology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt.
Samir F; Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt.
Nasr S; Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute (TBRI), Giza, Egypt.

Saudi J Biol Sci ; 29(5): 3097-3106, 2022 May.

Article in En | MEDLINE | ID: mdl-35360502

Key words

AMR, antimicrobial resistance; CLSI, clinical and laboratory standards institute; Caudovirales; ESKAPE, (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species); FOX, Cefoxitin; ITS, Internal transcribed spacer; LB, Luria-Bertani; Lytic bacteriophages; MDR, Multidrug-resistant; MRSA; MRSA, Methicillin Resistant Staphylococcus aureus; MitC, mitomycin C; Mitomycin C; NGS, double-stranded, ds, next generation sequencing; OX, Oxacillin; PCR, Polymerase chain reaction; PFU, Plaque forming unit; PTA, phosphotungstic acid; Polymerase chain reaction; Restriction digestion; S. aureus, Staphylococcus aureus; SDS-polyacrylamide gel electrophoresis; Sewage; TBRI, Theodor Bilharz research Institute; TEM, Transmission electron microscopy; Transmission electron microscopy

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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Saudi J Biol Sci Year: 2022 Document type: Article Affiliation country: Country of publication:

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