Ultra-trace enriching biosensing in nanoliter sample.

ABSTRACT

Rapid detection and accurate analysis of trace samples is an important prerequisite for precision medicine. Here we integrated capillary with ultrasound to induce biomarkers enrichment in nanoliter samples, and developed a nanoliter sample enrichment analysis method for ultra-trace miRNA biosensing. The interaction between ultrasonic field and capillary provides a gradient ultrasound field, which is essential for the aggregation of functionalized microspheres along with the enrichment of specific biomarkers. The results indicated that the enrichment of the biomarkers effectively enhanced the fluorescence intensity, and the limit of detection reaches 7.8✕10-12 M in 100 nL. Such integrated device can realize ultrasonic enrichment and visual analysis of target samples, and provides a new idea for rapid and highly sensitive detection of ultra-trace biomarkers in clinical diagnosis.