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[Evaluation of genetic diversity of ginseng fruit color germplasm resources: based on SSR analysis].
Qiao, Ping; Wang, Yi-Heng; Sun, Jia-Hui; Kang, Chuan-Zhi; Guo, Lan-Ping; Huang, Lu-Qi.
Affiliation
  • Qiao P; Academician Workstation, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Wang YH; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Sun JH; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Kang CZ; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Guo LP; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
  • Huang LQ; Academician Workstation, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi ; 47(8): 2158-2164, 2022 Apr.
Article in Zh | MEDLINE | ID: mdl-35531731
Illumina Xten was employed for shallow sequencing of Panax ginseng(ginseng) samples, MISA for screening of SSR loci, and Primer 3 for primer design. Polymorphic primers were screened from 180 primers. From the successfully amplified polymorphic primers, 15 primers which featured clear peak shape, good polymorphism, and ease of statistics were selected and used to evaluate the genetic diversity and germplasm resources of 36 ginseng accessions with different fruit colors from Jilin province. The results showed that red-fruit ginseng population had high genetic diversity with the average number of alleles(N_a) of 1.031 and haploid genetic diversity(h) of 0.172. The neighbor-joining cluster analysis demonstrated that the germplasms of red-fruit and yellow-fruit ginseng populations were obviously intermixed, and pick-fruit ginseng germplasms clustered into a single clade. The results of STRUCTURE analysis showed high proportion of single genotype in pick-fruit ginseng germplasm and abundant genotypes in red-fruit and yellow-fruit ginseng germplasms with obvious germplasm mixing. AMOVA revealed that genetic variation occurred mainly within populations(62.00%, P<0.001), and rarely among populations(39%, P<0.001), but homogenization was obvious among different populations. In summary, pink-fruit ginseng population may contain rare genotypes, which is the basis for breeding of high-quality high-yield, and multi-resistance varieties, genetic improvement of varieties, and sustainable development and utilization of ginseng germplasm resources.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microsatellite Repeats / Panax Language: Zh Journal: Zhongguo Zhong Yao Za Zhi Journal subject: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Year: 2022 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microsatellite Repeats / Panax Language: Zh Journal: Zhongguo Zhong Yao Za Zhi Journal subject: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Year: 2022 Document type: Article Affiliation country: Country of publication: