Isolation, identification, virulence potential and genomic features of Tenacibaculum piscium isolates recovered from Chilean salmonids.
Transbound Emerg Dis
; 69(5): e3305-e3315, 2022 Sep.
Article
in En
| MEDLINE
| ID: mdl-35674219
ABSTRACT
Tenacibaculum piscium, a gram-negative bacterium isolated from the skin ulcers of sea-farmed fish, has only been described in Norway. In the present study, we examined 16 Chilean Tenacibaculum isolates recovered from different organs in moribund and dead Atlantic salmon (Salmo salar), Rainbow trout (Oncorhynchus mykiss) and Coho salmon (Oncorhynchus kisutch) cultured at different fish farms between 2014 and 2018. The present study applied biochemical, phenotypic, fatty acid and whole-genome sequence-based analyses to confirm the taxonomic status of the Chilean isolates. The obtained results are the first to confirm the presence of T. piscium in Chile and in Coho salmon, thus extending the recognized geographical and species distribution of this bacterium. Subsequent bath-challenge assays in Atlantic salmon utilizing three T. piscium isolates obtained from different hosts resulted in low cumulative mortality (i.e. 0-35%), even after exposure to an unnaturally high concentration of bacterial cells (i.e. > 107 cells/ml). However, scale loss and frayed fins were observed in dead fish. In silico whole-genome analysis detected various genes associated with iron acquisition, encoding of the type IX secretion system and cargo proteins, resistance to tetracycline and fluoroquinolones and stress responses. These data represent an important milestone towards a better understanding on the genomic repertoire of T. piscium.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oncorhynchus mykiss
/
Oncorhynchus kisutch
/
Tenacibaculum
/
Fish Diseases
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
America do sul
/
Chile
Language:
En
Journal:
Transbound Emerg Dis
Journal subject:
MEDICINA VETERINARIA
Year:
2022
Document type:
Article
Affiliation country: