Au NP-Decorated g-C3N4-Based Photoelectochemical Biosensor for Sensitive Mercury Ions Analysis.

ABSTRACT

Herein, an efficient and feasible photoelectrochemical (PEC) biosensor based on gold nanoparticle-decorated graphitic-like carbon nitride (Au NPs@g-C3N4) with excellent photoelectric performance was designed for the highly sensitive detection of mercury ions (Hg2+) . The proposed Au NPs@g-C3N4 was first modified on the surface of the electrode, which possessed a remarkable photocurrent conversion efficiency and could produce a strong initial photocurrent. Then, the thymine-rich DNA (S1) was immobilized on the surface of the modified electrode via Au-N bonds. Subsequently, 1-hexanethiol (HT) was added to the resultant electrode to block nonspecific binding sites. Finally, the target Hg2+ was incubated on the surface of the modified glassy carbon electrode (GCE). In the presence of target Hg2+, the thymine-Hg2+-thymine (T-Hg2+-T) structure formed due to the selective capture capability of thymine base pairs toward Hg2+, resulting in the significantly decrease of the photocurrent. Thereafter, the proposed PEC biosensor was successfully used for sensitive Hg2+ detection, as it possessed a wide linear range from 1 pM to 1000 nM with a low detection limit of 0.33 pM. Importantly, this study demonstrates a new method of detecting Hg2+ and provides a promising platform for the detection of other heavy metal ions of interest.