Clinical and molecular change induced by repeated low-dose visible light exposure in both light-skinned and dark-skinned individuals.
Photodermatol Photoimmunol Photomed
; 39(3): 204-212, 2023 May.
Article
in En
| MEDLINE
| ID: mdl-35861041
ABSTRACT
BACKGROUND:
Visible light (VL) is known to induce pigmentation in dark-skinned individuals and immediate erythema in light-skinned individuals. However, the effects of accumulated low-dose VL exposure across skin types are not well established.METHODS:
Thirty-one healthy subjects with light (Fitzpatrick skin types [FST] I-II, n = 13) and dark (FST V-VI, n = 18) skin types were enrolled. Subjects' buttocks were exposed daily to VL, wavelength 400-700 nm, with a dose of 120 J/cm2 at 50 mW/cm2 , for four consecutive days. Microarray using Affymetrix GeneChip (49,395 genes) was performed followed by qRT-PCR on skin samples.RESULTS:
Repeated low-dose VL irradiation induced immediate pigment darkening and delayed tanning in dark-skinned individuals while no discernable pigmentation and erythema were observed in light-skinned individuals. Top ten upregulated genes by repeated VL exposure in microarray included melanogenic genes such as tyrosinase (TYR), tyrosinase-related protein-1 (TYRP1), dopachrome tautomerase (DCT), premelanosome protein (PMEL), melan-A (MLANA), and solute carrier family 24, member 5 (SLC24A5) and genes involved in inflammation/matrix remodeling/cell signaling including chemokine (C-C motif) ligand 18 (CCL18), BCL2-related protein A1 (BCL2A1), and cartilage oligomeric matrix protein (COMP). In qRT-PCR CCL18 was upregulated in light skin with a greater extent (mean fold change ± SD; 4.03 ± 3.28, p = .04) than in dark-skinned individuals (1.91 ± 1.32, p = .07) while TYR was not significantly upregulated in both skin types.CONCLUSION:
This study highlights the genes upregulated by cumulative VL exposure involved in pigmentation, immune response, oxidation/reduction, and matrix remodeling across skin types providing relevant information on daily solar exposure.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ultraviolet Rays
/
Skin Pigmentation
Limits:
Humans
Language:
En
Journal:
Photodermatol Photoimmunol Photomed
Journal subject:
ALERGIA E IMUNOLOGIA
/
DERMATOLOGIA
Year:
2023
Document type:
Article
Affiliation country: