Optimizing CRISPR/Cas9-based gene manipulation in echinoderms.
Dev Biol
; 490: 117-124, 2022 10.
Article
in En
| MEDLINE
| ID: mdl-35917936
ABSTRACT
The impact of new technology can be appreciated by how broadly it is used. Investigators that previously relied only on pharmacological approaches or the use of morpholino antisense oligonucleotide (MASO) technologies are now able to apply CRISPR-Cas9 to study biological problems in their model organism of choice much more effectively. The transitions to new CRISPR-based approaches could be enhanced, first, by standardized protocols and education in their applications. Here we summarize our results for optimizing the CRISPR-Cas9 technology in a sea urchin and a sea star, and provide advice on how to set up CRISPR-Cas9 experiments and interpret the results in echinoderms. Our goal through these protocols and sharing examples of success by other labs is to lower the activation barrier so that more laboratories can apply CRISPR-Cas9 technologies in these important animals.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sea Urchins
/
CRISPR-Cas Systems
Limits:
Animals
Language:
En
Journal:
Dev Biol
Year:
2022
Document type:
Article
Affiliation country: