Your browser doesn't support javascript.
loading
A novel microRNA signature for the detection of melanoma by liquid biopsy.
Sabato, Claudia; Noviello, Teresa Maria Rosaria; Covre, Alessia; Coral, Sandra; Caruso, Francesca Pia; Besharat, Zein Mersini; Splendiani, Elena; Masuelli, Laura; Battistelli, Cecilia; Vacca, Alessandra; Catanzaro, Giuseppina; Po, Agnese; Anichini, Andrea; Maio, Michele; Ceccarelli, Michele; Di Giacomo, Anna Maria; Ferretti, Elisabetta.
Affiliation
  • Sabato C; Department of Experimental Medicine, Sapienza University, 00161, Rome, Italy.
  • Noviello TMR; Biogem Scarl, Istituto di Ricerche Genetiche "Gaetano Salvatore", 83031, Ariano Irpino, Italy.
  • Covre A; Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy.
  • Coral S; Center for Immuno-Oncology, Medical Oncology and Immunotherapy, Department of Oncology, University Hospital of Siena, 53100, Siena, Italy.
  • Caruso FP; Medical Oncology, Department of Molecular and Developmental Medicine, University of Siena, 53100, Siena, Italy.
  • Besharat ZM; Center for Immuno-Oncology, Medical Oncology and Immunotherapy, Department of Oncology, University Hospital of Siena, 53100, Siena, Italy.
  • Splendiani E; Epigen Therapeutics s.r.l., 53100, Siena, Italy.
  • Masuelli L; Biogem Scarl, Istituto di Ricerche Genetiche "Gaetano Salvatore", 83031, Ariano Irpino, Italy.
  • Battistelli C; Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy.
  • Vacca A; Department of Experimental Medicine, Sapienza University, 00161, Rome, Italy.
  • Catanzaro G; Department of Molecular Medicine, Sapienza University, 00161, Rome, Italy.
  • Po A; Department of Experimental Medicine, Sapienza University, 00161, Rome, Italy.
  • Anichini A; Department of Molecular Medicine, Sapienza University, 00161, Rome, Italy.
  • Maio M; Department of Experimental Medicine, Sapienza University, 00161, Rome, Italy.
  • Ceccarelli M; Department of Experimental Medicine, Sapienza University, 00161, Rome, Italy.
  • Di Giacomo AM; Department of Molecular Medicine, Sapienza University, 00161, Rome, Italy.
  • Ferretti E; Human Tumor Immunobiology Unit, Department of Research, Fondazione IRCCS Istituto Nazionale dei Tumori, ENETS Center of Excellence, Milan, Italy.
J Transl Med ; 20(1): 469, 2022 10 15.
Article in En | MEDLINE | ID: mdl-36243798
ABSTRACT

BACKGROUND:

Melanoma is the deadliest form of skin cancer and metastatic disease is associated with a significant survival rate drop. There is an urgent need for consistent tumor biomarkers to scale precision medicine and reduce cancer mortality. Here, we aimed to identify a melanoma-specific circulating microRNA signature and assess its value as a diagnostic tool.

METHODS:

The study consisted of a discovery phase and two validation phases. Circulating plasma extracellular vesicles (pEV) associated microRNA profiles were obtained from a discovery cohort of metastatic melanoma patients and normal subjects as controls. A pEV-microRNA signature was obtained using a LASSO penalized logistic regression model. The pEV-microRNA signature was subsequently validated both in a publicly available dataset and in an independent internal cohort.

RESULTS:

We identified and validated in three independent cohorts a panel of melanoma-specific circulating microRNAs that showed high accuracy in differentiating melanoma patients from healthy subjects with an area under the curve (AUC) of 1.00, 0.94 and 0.75 respectively. Investigation of the function of the pEV-microRNA signature evidenced their possible immune suppressive role in melanoma patients.

CONCLUSIONS:

We demonstrate that a blood test based on circulating microRNAs can non-invasively detect melanoma, offering a novel diagnostic tool for improving standard care. Moreover, we revealed an immune suppressive role for melanoma pEV-microRNAs.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / Circulating MicroRNA / Melanoma Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: J Transl Med Year: 2022 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / Circulating MicroRNA / Melanoma Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: J Transl Med Year: 2022 Document type: Article Affiliation country: