The Accessory Limb Model Regenerative Assay and Its Derivatives.
Methods Mol Biol
; 2562: 217-233, 2023.
Article
in En
| MEDLINE
| ID: mdl-36272079
ABSTRACT
When the Accessory Limb Model (ALM) regenerative assay was first published by Endo, Bryant, and Gardiner in 2004, it provided a robust system for testing the cellular and molecular contributions during each of the basic steps of regeneration the formation of the wound epithelium, neural induction of the apical epithelial cap, and the formation of a positional disparity between blastema cells. The basic ALM procedure was developed in the axolotl and involves deviating a limb nerve into a lateral wound and grafting skin from the opposing side of the limb axis into the site of injury. In this chapter, we will review the studies that lead to the conception of the ALM, as well as the studies that have followed the development of this assay. We will additionally describe in detail the standard ALM surgery and how to perform this surgery on different limb positions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ambystoma mexicanum
/
Nerve Tissue
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2023
Document type:
Article
Affiliation country: