Generation of induced pluripotent stem cells from three individuals with Huntington's disease.
Stem Cell Res
; 65: 102976, 2022 12.
Article
in En
| MEDLINE
| ID: mdl-36434993
ABSTRACT
Huntington's disease (HD) is a neurodegenerative disorder caused by abnormal glutamine (Q) expansion in the huntingtin protein due to elongated CAG repeats in the gene HTT. We used non-integrative episomal plasmids to generate induced pluripotent stem cells (iPSCs) from three individuals affected by HD CH1 (58Q), and two twin brothers CH3 (44Q) and CH4 (44Q). The iPSC lines exhibited one healthy HTT allele and one with elongated CAG repeats, as confirmed by PCR and sequencing. All iPSC lines expressed pluripotency markers, exhibited a normal karyotype, and generated cells of the three germ layers in vitro.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Huntington Disease
/
Induced Pluripotent Stem Cells
/
Huntingtin Protein
Limits:
Humans
/
Male
Language:
En
Journal:
Stem Cell Res
Year:
2022
Document type:
Article
Affiliation country: