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Generation of induced pluripotent stem cells from three individuals with Huntington's disease.
Miller, Duncan C; Lisowski, Pawel; Lickfett, Selene; Mlody, Barbara; Bünning, Miriam; Genehr, Carolin; Ulrich, Claas; Wanker, Erich E; Diecke, Sebastian; Priller, Josef; Prigione, Alessandro.
Affiliation
  • Miller DC; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany.
  • Lisowski P; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Magdalenka n/Warsaw, Poland; Neurops
  • Lickfett S; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany.
  • Mlody B; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany.
  • Bünning M; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany.
  • Genehr C; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany.
  • Ulrich C; Department of Dermatology and Allergy, Charité - Universitätsmedizin Berlin, Germany.
  • Wanker EE; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany.
  • Diecke S; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany. Electronic address: Sebastian.diecke@mdc-berlin.de.
  • Priller J; Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité - Universitätsmedizin Berlin, Germany; DZNE, Berlin, Germany; University of Edinburgh and UK DRI, Edinburgh, UK; Department of Psychiatry and Psychotherapy, School of Medicine, Technical University Munich, Germany. Electronic address: j
  • Prigione A; Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany. Electronic address: alessandro.prigione@hhu.de.
Stem Cell Res ; 65: 102976, 2022 12.
Article in En | MEDLINE | ID: mdl-36434993
ABSTRACT
Huntington's disease (HD) is a neurodegenerative disorder caused by abnormal glutamine (Q) expansion in the huntingtin protein due to elongated CAG repeats in the gene HTT. We used non-integrative episomal plasmids to generate induced pluripotent stem cells (iPSCs) from three individuals affected by HD CH1 (58Q), and two twin brothers CH3 (44Q) and CH4 (44Q). The iPSC lines exhibited one healthy HTT allele and one with elongated CAG repeats, as confirmed by PCR and sequencing. All iPSC lines expressed pluripotency markers, exhibited a normal karyotype, and generated cells of the three germ layers in vitro.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Huntington Disease / Induced Pluripotent Stem Cells / Huntingtin Protein Limits: Humans / Male Language: En Journal: Stem Cell Res Year: 2022 Document type: Article Affiliation country:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Huntington Disease / Induced Pluripotent Stem Cells / Huntingtin Protein Limits: Humans / Male Language: En Journal: Stem Cell Res Year: 2022 Document type: Article Affiliation country: