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Morphological Evidence for Novel Roles of Microtubules in Macrophage Phagocytosis.
Seta, Yoshika; Kawakatsu, Kumpei; Degawa, Shiori; Goto, Toshiyuki; Nishikata, Takahito.
Affiliation
  • Seta Y; Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Minatojima-Minamimachi, Chuo-ku, Kobe 605-0047, Japan.
  • Kawakatsu K; Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Minatojima-Minamimachi, Chuo-ku, Kobe 605-0047, Japan.
  • Degawa S; Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Minatojima-Minamimachi, Chuo-ku, Kobe 605-0047, Japan.
  • Goto T; Laboratory Cellular Function Imaging, RIKEN Center for Biosystems Dynamics Research (BDR), Minatojima-Minamimachi, Chuo-ku, Kobe 605-0047, Japan.
  • Nishikata T; Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Minatojima-Minamimachi, Chuo-ku, Kobe 605-0047, Japan.
Int J Mol Sci ; 24(2)2023 Jan 10.
Article in En | MEDLINE | ID: mdl-36674886
Although the phagocytic activity of macrophages has long been studied, the involvement of microtubules in the process is not well understood. In this study, we improved the fixation protocol and revealed a dynamically rearranging microtubule network in macrophages, consisting of a basal meshwork, thick bundles at the cell edge, and astral microtubules. Some astral microtubules extended beneath the cell cortex and continued to form bundles at the cell edge. These microtubule assemblies were mutually exclusive of actin accumulation during membrane ruffling. Although the stabilization of microtubules with paclitaxel did not affect the resting stage of the macrophages, it reduced the phagocytic activity and membrane ruffling of macrophages activated with serum-MAF, which induced rapid phagocytosis. In contrast, the destabilization of microtubules with nocodazole enhanced membrane ruffling and the internalization of phagocytic targets suggesting an inhibitory effect of the microtubule network on the remodeling of the actin network. Meanwhile, the microtubule network was necessary for phagosome maturation. Our detailed analyses of cytoskeletal filaments suggest a phagocytosis control system involving Ca2+ influx, the destabilization of microtubules, and activation of actin network remodeling, followed by the translocation and acidification of phagosomes on the microtubule bundles.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phagocytosis / Actins Language: En Journal: Int J Mol Sci Year: 2023 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phagocytosis / Actins Language: En Journal: Int J Mol Sci Year: 2023 Document type: Article Affiliation country: Country of publication: