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An Environmental DNA Primer for Microbial and Restoration Ecology.
Tessler, Michael; Cunningham, Seth W; Ingala, Melissa R; Warring, Sally D; Brugler, Mercer R.
Affiliation
  • Tessler M; Department of Biology, St. Francis College, Brooklyn, NY, USA. mtessler@sfc.edu.
  • Cunningham SW; Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY, 10024, USA. mtessler@sfc.edu.
  • Ingala MR; Division of Invertebrate Zoology, American Museum of Natural History, New York, NY, 10024, USA. mtessler@sfc.edu.
  • Warring SD; Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, NY, 10024, USA.
  • Brugler MR; Department of Biological Sciences, Fordham University, Bronx, NY, 10458, USA.
Microb Ecol ; 85(3): 796-808, 2023 Apr.
Article in En | MEDLINE | ID: mdl-36735064
Environmental DNA (eDNA) sequencing-DNA collected from the environment from living cells or shed DNA-was first developed for working with microbes and has greatly benefitted microbial ecologists for decades since. These tools have only become increasingly powerful with the advent of metabarcoding and metagenomics. Most new studies that examine diverse assemblages of bacteria, archaea, protists, fungi, and viruses lean heavily into eDNA using these newer technologies, as the necessary sequencing technology and bioinformatic tools have become increasingly affordable and user friendly. However, eDNA methods are rapidly evolving, and sometimes it can feel overwhelming to simply keep up with the basics. In this review, we provide a starting point for microbial ecologists who are new to DNA-based methods by detailing the eDNA methods that are most pertinent, including study design, sample collection and storage, selecting the right sequencing technology, lab protocols, equipment, and a few bioinformatic tools. Furthermore, we focus on how eDNA work can benefit restoration and what modifications are needed when working in this subfield.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Environmental Language: En Journal: Microb Ecol Year: 2023 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Environmental Language: En Journal: Microb Ecol Year: 2023 Document type: Article Affiliation country: Country of publication: