[Repair of mandibular defects with hydrogel loaded with nano-hydroxyapatite in rats].
Shanghai Kou Qiang Yi Xue
; 31(5): 449-453, 2022 Oct.
Article
in Zh
| MEDLINE
| ID: mdl-36758589
ABSTRACT
PURPOSE:
To observe the effect of gellan gum loaded with nano-hydroxyapatite (GG/nHA) on repairing mandibular defect in rats.METHODS:
Critical bone defects with 5 mm in diameter on the mandible of 16 SD rats were created and randomly divided into two groups. The bone defects in the experimental group were injected with GG/nHA and the control group were filled with absorbable gelatin sponge. The rats were sacrificed at 4 and 8 weeks after operation. The bone tissue healing was evaluated by Micro-CT. Bone tissue repairing effect was evaluated by hematoxylin-eosin (H-E) staining and Masson staining. GraphPad Prism 8.0 software package was used for statistical analysis.RESULTS:
The prepared GG/nHA had a good injectability and could be delivered to the bone defect area with a syringe. Four and 8 weeks after operation, the newly formed bone and bone volume fraction (BV/TV) in the experimental group were higher than those in the control group, and the difference was statistically significant (P<0.05). Larger number of new bone were observed in the experimental group than the control group by H-E staining and Masson staining.CONCLUSIONS:
GG/nHA can be injected into the mandibular defect area to promote its healing, and it is expected to be used as a novel bio-material for minimally invasive repair of oral and maxillofacial bone defects.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bone Regeneration
/
Durapatite
Limits:
Animals
Language:
Zh
Journal:
Shanghai Kou Qiang Yi Xue
Journal subject:
ODONTOLOGIA
Year:
2022
Document type:
Article