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Three dimensional models of dedifferentiated liposarcoma cell lines: scaffold-based and scaffold-free approaches.
Tahara, Sayumi; de Faria, Fernanda Costas C; Sarchet, Patricia; Calore, Federica; Sharick, Joe; Leight, Jennifer L; Casadei, Lucia; Pollock, Raphael E.
Affiliation
  • Tahara S; Comprehensive Cancer Center, The Ohio State University, 460 West 10th Avenue. Suite D920E, Columbus, OH, 43210, USA.
  • de Faria FCC; Comprehensive Cancer Center, The Ohio State University, 460 West 10th Avenue. Suite D920E, Columbus, OH, 43210, USA.
  • Sarchet P; Comprehensive Cancer Center, The Ohio State University, 460 West 10th Avenue. Suite D920E, Columbus, OH, 43210, USA.
  • Calore F; Comprehensive Cancer Center, The Ohio State University, 460 West 10th Avenue. Suite D920E, Columbus, OH, 43210, USA.
  • Sharick J; Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH, USA.
  • Leight JL; Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH, USA.
  • Casadei L; Comprehensive Cancer Center, The Ohio State University, 460 West 10th Avenue. Suite D920E, Columbus, OH, 43210, USA.
  • Pollock RE; Comprehensive Cancer Center, The Ohio State University, 460 West 10th Avenue. Suite D920E, Columbus, OH, 43210, USA. raphael.pollock@osumc.edu.
Hum Cell ; 36(3): 1081-1089, 2023 May.
Article in En | MEDLINE | ID: mdl-36763259
ABSTRACT
Sarcomas are rare malignancies, the number of reports is limited, and this rarity makes further research difficult even though liposarcoma is one of major sarcomas. 2D cell culture remains an important role in establishing basic tumor biology research, but its various shortcomings and limitations are still of concern, and it is now well-accepted that the behavior of 3D-cultured cells is more reflective of in vivo cellular responses compared to 2D models. This study aimed to establish 3D cell culture of liposarcomas using two different

methods:

scaffold-based (Matrigel extracellular matrix [ECM] scaffold method) and scaffold-free (Ultra-low attachment [ULA] plate). Lipo246, Lipo224 and Lipo863 cell lines were cultured, and distinctive differences in structures were observed in Matrigel 3D model Lipo224 and Lipo863 formed spheroids, whereas Lipo246 grew radially without forming spheres. In ULA plate approaches, all cell lines formed spheroids, but Lipo224 and Lipo863 spheroids showed bigger size and looser aggregation than Lipo246. Formalin fixed, paraffin embedded (FFPE) blocks were obtained from all 3D models, confirming the spheroid structures. The expression of MDM2, Ki-67 positivity and MDM2 amplification were confirmed by IHC and DNAscope™, respectively. Protein and DNA were extracted from all samples and MDM2 upregulation was confirmed by western blot and qPCR analysis. After treatment with MDM2 inhibitor SAR405838, DDLPS spheroids demonstrated different sensitivity patterns from 2D models. Taken together, we believed that 3D models would have a possibility to provide us a new predictability of efficacy and toxicity, and considered as one important process in in vitro pre-clinical phase prior to moving forward to clinical trials.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sarcoma / Soft Tissue Neoplasms / Liposarcoma Type of study: Prognostic_studies Limits: Humans Language: En Journal: Hum Cell Year: 2023 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sarcoma / Soft Tissue Neoplasms / Liposarcoma Type of study: Prognostic_studies Limits: Humans Language: En Journal: Hum Cell Year: 2023 Document type: Article Affiliation country: