CRISPR-Cas9 Methods and Key Considerations in the Production of Aedes aegypti Mutant Strains.
Cold Spring Harb Protoc
; 2023(9): 607-613, 2023 09 01.
Article
in En
| MEDLINE
| ID: mdl-36931732
ABSTRACT
Since the characterization of the CRISPR-Cas9 system in prokaryotes, it has become the prime choice in gene editing because of its exceptional flexibility, ease of use, high efficiency, and superior specificity. As a result, CRISPR-Cas9-mediated gene-editing technologies have enabled researchers not only to engineer transgenic animal strains with site-directed insertions more efficiently but also to generate desired mutants for previously intractable species. One such species is the invasive yellow fever mosquito, Aedes aegypti, which is notorious for its ability to transmit many blood-borne human pathogens. Methods for developing new transgenic strains of the yellow fever mosquito may aid in the effort to control its populations and provide significant benefits for the public. Here, we provide an overview of injection and noninjection methods for generating transgenic mosquitoes and also highlight important experimental design features.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Yellow Fever
/
Aedes
Limits:
Animals
/
Humans
Language:
En
Journal:
Cold Spring Harb Protoc
Year:
2023
Document type:
Article
Affiliation country: