Multi-tissue DNA methylation aging clocks for sea lions, walruses and seals.
Commun Biol
; 6(1): 359, 2023 04 01.
Article
in En
| MEDLINE
| ID: mdl-37005462
ABSTRACT
Age determination of wild animals, including pinnipeds, is critical for accurate population assessment and management. For most pinnipeds, current age estimation methodologies utilize tooth or bone sectioning which makes antemortem estimations problematic. We leveraged recent advances in the development of epigenetic age estimators (epigenetic clocks) to develop highly accurate pinniped epigenetic clocks. For clock development, we applied the mammalian methylation array to profile 37,492 cytosine-guanine sites (CpGs) across highly conserved stretches of DNA in blood and skin samples (n = 171) from primarily three pinniped species representing the three phylogenetic families Otariidae, Phocidae and Odobenidae. We built an elastic net model with Leave-One-Out-Cross Validation (LOOCV) and one with a Leave-One-Species-Out-Cross-Validation (LOSOCV). After identifying the top 30 CpGs, the LOOCV produced a highly correlated (r = 0.95) and accurate (median absolute error = 1.7 years) age estimation clock. The LOSOCV elastic net results indicated that blood and skin clock (r = 0.84) and blood (r = 0.88) pinniped clocks could predict age of animals from pinniped species not used for clock development to within 3.6 and 4.4 years, respectively. These epigenetic clocks provide an improved and relatively non-invasive tool to determine age in skin or blood samples from all pinniped species.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Caniformia
/
Sea Lions
/
Seals, Earless
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Commun Biol
Year:
2023
Document type:
Article
Affiliation country: