Combining on-line spectroscopy with synchrotron and X-ray free electron laser crystallography.
Curr Opin Struct Biol
; 80: 102604, 2023 06.
Article
in En
| MEDLINE
| ID: mdl-37148654
ABSTRACT
With the recent advances in serial crystallography methods at both synchrotron and X-ray free electron laser sources, more details of intermediate or transient states of the catalytic reactions are being revealed structurally. These structural studies of reaction dynamics drive the need for on-line in crystallo spectroscopy methods to complement the crystallography experiment. The recent applications of combined spectroscopy and crystallography methods enable on-line determination of in crystallo reaction kinetics and structures of catalytic intermediates, sample integrity, and radiation-induced sample modifications, if any, as well as heterogeneity of crystals from different preparations or sample batches. This review describes different modes of spectroscopy that are combined with the crystallography experiment at both synchrotron and X-ray free-electron laser facilities, and the complementary information that each method can provide to facilitate the structural study of enzyme catalysis and protein dynamics.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Synchrotrons
/
Electrons
Language:
En
Journal:
Curr Opin Struct Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2023
Document type:
Article
Affiliation country: