Your browser doesn't support javascript.
loading
High-throughput RNA isoform sequencing using programmed cDNA concatenation.
Al'Khafaji, Aziz M; Smith, Jonathan T; Garimella, Kiran V; Babadi, Mehrtash; Popic, Victoria; Sade-Feldman, Moshe; Gatzen, Michael; Sarkizova, Siranush; Schwartz, Marc A; Blaum, Emily M; Day, Allyson; Costello, Maura; Bowers, Tera; Gabriel, Stacey; Banks, Eric; Philippakis, Anthony A; Boland, Genevieve M; Blainey, Paul C; Hacohen, Nir.
Affiliation
  • Al'Khafaji AM; Broad Institute of MIT and Harvard, Cambridge, MA, USA. aalkhafa@broadinstitute.org.
  • Smith JT; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Garimella KV; Broad Institute of MIT and Harvard, Cambridge, MA, USA. kiran@broadinstitute.org.
  • Babadi M; Broad Institute of MIT and Harvard, Cambridge, MA, USA. mehrtash@broadinstitute.org.
  • Popic V; Broad Institute of MIT and Harvard, Cambridge, MA, USA. vpopic@broadinstitute.org.
  • Sade-Feldman M; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Gatzen M; Department of Medicine, Center for Cancer Research, Massachusetts General Hospital, Boston, MA, USA.
  • Sarkizova S; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Schwartz MA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Blaum EM; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Day A; Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
  • Costello M; Division of Hematology/Oncology, Boston Children's Hospital, Boston, MA, USA.
  • Bowers T; Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA, USA.
  • Gabriel S; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Banks E; Department of Medicine, Center for Cancer Research, Massachusetts General Hospital, Boston, MA, USA.
  • Philippakis AA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Boland GM; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Blainey PC; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Hacohen N; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Nat Biotechnol ; 42(4): 582-586, 2024 Apr.
Article in En | MEDLINE | ID: mdl-37291427
ABSTRACT
Full-length RNA-sequencing methods using long-read technologies can capture complete transcript isoforms, but their throughput is limited. We introduce multiplexed arrays isoform sequencing (MAS-ISO-seq), a technique for programmably concatenating complementary DNAs (cDNAs) into molecules optimal for long-read sequencing, increasing the throughput >15-fold to nearly 40 million cDNA reads per run on the Sequel IIe sequencer. When applied to single-cell RNA sequencing of tumor-infiltrating T cells, MAS-ISO-seq demonstrated a 12- to 32-fold increase in the discovery of differentially spliced genes.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: High-Throughput Nucleotide Sequencing / RNA Isoforms Language: En Journal: Nat Biotechnol Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article Affiliation country:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: High-Throughput Nucleotide Sequencing / RNA Isoforms Language: En Journal: Nat Biotechnol Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article Affiliation country: