Your browser doesn't support javascript.
loading
Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells.
Stephens, Dominique C; Crabtree, Amber; Beasley, Heather K; Garza-Lopez, Edgar; Neikirk, Kit; Mungai, Margaret; Vang, Larry; Vue, Zer; Vue, Neng; Marshall, Andrea G; Turner, Kyrin; Shao, Jianqiang; Murray, Sandra; Gaddy, Jennifer A; Wanjalla, Celestine; Davis, Jamaine; Damo, Steven; Hinton, Antentor O.
Affiliation
  • Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Crabtree A; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA.
  • Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Garza-Lopez E; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Neikirk K; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
  • Mungai M; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Vang L; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
  • Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Vue N; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Turner K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
  • Shao J; Department of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA.
  • Murray S; Central Microscopy Research Facility, University of Iowa, Iowa City, IA, 52242, USA.
  • Gaddy JA; Department of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, PA, 15260, USA.
  • Wanjalla C; Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232 USA.
  • Davis J; Tennessee Valley Healthcare Systems, U.S. Department of Veterans Affairs, Nashville, Tennessee, 37212 USA.
  • Damo S; Vanderbilt University Medical Center: Department of Medicine, Division of Infectious Disease, Nashville, TN, USA.
  • Hinton AO; Department of Biochemistry and Cancer Biology. Meharry Medical College, Nashville, TN, 37208, USA.
bioRxiv ; 2023 Aug 19.
Article in En | MEDLINE | ID: mdl-37292700
ABSTRACT
Proximity ligation assays (PLA) use specific antibodies to detect endogenous protein-protein interactions. PLA is a highly useful biochemical technique that allows two proteins within close proximity to be visualized with fluorescent probes amplified by PCR. While this technique has gained prominence, the use of PLA in mouse skeletal muscle (SkM) is novel. In this article, we discuss how the PLA method can be used in SkM to study the protein-protein interactions within mitochondria-endoplasmic reticulum contact sites (MERCs).
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: BioRxiv Year: 2023 Document type: Article Affiliation country:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: BioRxiv Year: 2023 Document type: Article Affiliation country: