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Novel Amplicon-Based Sequencing Approach to West Nile Virus.
Diagne, Moussa Moïse; Ndione, Marie Henriette Dior; Mencattelli, Giulia; Diallo, Amadou; Ndiaye, El Hadji; Di Domenico, Marco; Diallo, Diawo; Kane, Mouhamed; Curini, Valentina; Top, Ndeye Marieme; Marcacci, Maurilia; Mbanne, Maïmouna; Ancora, Massimo; Secondini, Barbara; Di Lollo, Valeria; Teodori, Liana; Leone, Alessandra; Puglia, Ilaria; Gaye, Alioune; Sall, Amadou Alpha; Loucoubar, Cheikh; Rosà, Roberto; Diallo, Mawlouth; Monaco, Federica; Faye, Ousmane; Cammà, Cesare; Rizzoli, Annapaola; Savini, Giovanni; Faye, Oumar.
Affiliation
  • Diagne MM; Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Ndione MHD; Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Mencattelli G; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Diallo A; Centre Agriculture Food Environment, University of Trento, 38010 San Michele all'Adige, Italy.
  • Ndiaye EH; Research and Innovation Centre, Fondazione Edmund Mach, 38010 San Michele all'Adige, Italy.
  • Di Domenico M; Epidemiology, Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Diallo D; Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Kane M; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Curini V; Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Top NM; Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Marcacci M; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Mbanne M; Epidemiology, Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Ancora M; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Secondini B; Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Di Lollo V; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Teodori L; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Leone A; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Puglia I; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Gaye A; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Sall AA; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Loucoubar C; Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Rosà R; Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Diallo M; Epidemiology, Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Monaco F; Centre Agriculture Food Environment, University of Trento, 38010 San Michele all'Adige, Italy.
  • Faye O; Medical Zoology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Cammà C; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Rizzoli A; Virology Department, Institut Pasteur de Dakar, Dakar BP220, Senegal.
  • Savini G; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, 64100 Teramo, Italy.
  • Faye O; Research and Innovation Centre, Fondazione Edmund Mach, 38010 San Michele all'Adige, Italy.
Viruses ; 15(6)2023 05 27.
Article in En | MEDLINE | ID: mdl-37376561
ABSTRACT
West Nile virus is a re-emerging arbovirus whose impact on public health is increasingly important as more and more epidemics and epizootics occur, particularly in America and Europe, with evidence of active circulation in Africa. Because birds constitute the main reservoirs, migratory movements allow the diffusion of various lineages in the world. It is therefore crucial to properly control the dispersion of these lineages, especially because some have a greater health impact on public health than others. This work describes the development and validation of a novel whole-genome amplicon-based sequencing approach to West Nile virus. This study was carried out on different strains from lineage 1 and 2 from Senegal and Italy. The presented protocol/approach showed good coverage using samples derived from several vertebrate hosts and may be valuable for West Nile genomic surveillance.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: West Nile Fever / West Nile virus Limits: Animals / Humans Country/Region as subject: Africa / Europa Language: En Journal: Viruses Year: 2023 Document type: Article Affiliation country:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: West Nile Fever / West Nile virus Limits: Animals / Humans Country/Region as subject: Africa / Europa Language: En Journal: Viruses Year: 2023 Document type: Article Affiliation country: