1H, 13C and 15N backbone and side-chain resonance assignments of ∆∆BmSA1, the surface antigen of Babesia microti.
Biomol NMR Assign
; 17(2): 217-221, 2023 12.
Article
in En
| MEDLINE
| ID: mdl-37452919
ABSTRACT
Human babesiosis is a vector-borne zoonotic infection caused mostly by the Apicomplexan parasite Babesia microti, distributed worldwide. The infection can result in severe symptoms such as hemolytic anemia, especially in immunodeficient patients. Also, asymptomatic patients continue transmission as unscreened blood donors, and represent a risk for Public Health. Early host-parasite interactions are mediated by BmSA1, the major surface antigen of Babesia microti, crucial for invasion and immune escape. Hence, a structural and functional characterization of the BmSA1 protein constitutes a first strategic milestone toward the development of innovative tools to control infection. Knowledge of the 3D structure of such an important antigen is crucial for the development of vaccines or new diagnostic tests. Here, we report the 1H, 15N and 13C NMR resonance assignment of ∆∆BmSA1, a truncated recombinant version of BmSA1 without the N-terminal signal peptide and the hydrophobic C-terminal GPI-anchor. Secondary structure prediction using CSI.3 and TALOS-N demonstrates a high content of alpha-helical structure. This preliminary study provides foundations for further structural characterization of BMSA1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Babesiosis
/
Babesia microti
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Biomol NMR Assign
Journal subject:
BIOLOGIA MOLECULAR
/
MEDICINA NUCLEAR
Year:
2023
Document type:
Article
Affiliation country: