Expression profiles of MMP-9 and EMMPRIN in chronic rhinosinusitis with nasal polyps.
Acta Otorhinolaryngol Ital
; 43(6): 400-408, 2023 Dec.
Article
in En
| MEDLINE
| ID: mdl-37519135
ABSTRACT
Objective:
Metalloproteinases (MMPs) are implicated in tissue remodeling in chronic rhinosinusitis with nasal polyps (CRSwNP). This study aimed to evaluate the expression profiles of MMP-9 and the extracellular matrix metalloproteinase inducer (EMMPRIN) in nasal polyps compared to healthy mucosa.Methods:
Tissue samples from 37 CRSwNP patients undergoing functional endoscopic sinus surgery and mucosal specimens from 12 healthy controls were obtained intra-operatively. MMP-9 and EMMPRIN mRNA levels were assessed by reverse transcription-polymerase chain reaction and their protein expression by Western blot analysis.Results:
MMP-9 mRNA expression levels were significantly elevated in CRSwNP compared to controls (p < 0.05). MMP-9 protein levels presented an increasing trend but with no statistical significance (p > 0.05). No statistically significant difference in EMMPRIN mRNA and protein levels was identified.Conclusions:
Upregulation of MMP-9 in nasal polyps is evident and highlights its role in the pathogenesis of CRSwNP. The lack of concordance between MMP-9 mRNA and protein levels may be attributed to post-translational gene expression regulation. Although EMMPRIN expression was not significantly different between the two groups, its role remains to be elucidated. MMP-9 may be a valuable biomarker and treatment target in CRSwNP.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nasal Polyps
/
Rhinosinusitis
Limits:
Humans
Language:
En
Journal:
Acta Otorhinolaryngol Ital
Year:
2023
Document type:
Article
Affiliation country: