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Biosynthesized silver nanoparticles (Ag NPs) from isolated actinomycetes strains and their impact on the black cutworm, Agrotis ipsilon.
Abou El-Enain, Inas M; Elqady, Enayat M; El-Said, Eman; Salem, Hend H A; Badr, Naglaa Fathi; Abd-Allah, Ghada E; Rezk, Mohamed M.
Affiliation
  • Abou El-Enain IM; Botany and Microbiology Dept., Faculty of Science, Al-Azhar University-Cairo, Cairo, Egypt.
  • Elqady EM; Zoology and Entomology Dept., Faculty of Science, Al-Azhar University-Cairo, Cairo, Egypt.
  • El-Said E; Zoology and Entomology Dept., Faculty of Science, Al-Azhar University-Cairo, Cairo, Egypt.
  • Salem HHA; Zoology and Entomology Dept., Faculty of Science, Al-Azhar University-Cairo, Cairo, Egypt.
  • Badr NF; Zoology and Entomology Dept., Faculty of Science, Al-Azhar University-Cairo, Cairo, Egypt.
  • Abd-Allah GE; Plant Protection Research Institute, Agriculture Research Center, Cairo, Egypt.
  • Rezk MM; Isotops Dept., Research Sector, Nuclear Materials Authority, Cairo, Egypt. Electronic address: DDrezk@Yahoo.com.
Pestic Biochem Physiol ; 194: 105492, 2023 Aug.
Article in En | MEDLINE | ID: mdl-37532318
Nanomaterials have been produced with the use of bio-nanotechnology, which is a low-cost approach. Currently, research is being conducted to determine whether actinomycetes isolated from Egyptian soil can biosynthesize Ag nanoparticles (Ag NPs) and characterized by using the following techniques: Transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier transforms infrared (FT-IR), Energy-dispersive X-ray spectroscopy (EDX), UV-Vis spectroscopy and X-ray diffraction (XRD). The most promising actinomycetes isolate were identified, morphologically, biochemically, and molecularly. Streptomyces avermitilis Azhar A.4 was found to be able to reduce silver metal nanoparticles from silver nitrate in nine isolates collected from Egyptian soil. Toxicity of biosynthesized against 2nd and 4th larval instar of Agrotis ipsilon (Hufn.) (Lepidoptera: Noctuidae) was estimated. In addition, activity of certain vital antioxidant and detoxifying enzymes as well as midgut histology of treated larvae were also investigated. The results showed appositive correlations between larval mortality percentage (y) and bio-AgNPs concentrations (x) with excellent (R2). The 4th larval instar was more susceptible than 2nd larval instar with LC50 (with 95% confirmed limits) =8.61 (2.76-13.89) and 26.44(13.25-35.58) ppml-1, respectively of 5 days from treatment. The initial stages of biosynthesized AgNps exposure showed significant increases in carboxylesterase (CarE) and peroxidases (PODs) activity followed by significant suppression after 5 days pos-exposure. While protease activity was significantly decreased by increasing time post-exposure. Midgut histology showed abnormality and progressive damage by increasing time post exposure leading to complete destruction of midgut cells after 5 days from exposure. These results make biosynthesized AgNPs an appropriate alternative to chemical insecticide in A. ipsilon management.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Actinobacteria / Metal Nanoparticles Limits: Animals Language: En Journal: Pestic Biochem Physiol Year: 2023 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Actinobacteria / Metal Nanoparticles Limits: Animals Language: En Journal: Pestic Biochem Physiol Year: 2023 Document type: Article Affiliation country: Country of publication: